The Right Tool for Every Scientific Masterpiece
Just like a painter selects the perfect brush, researchers choose the right tools to bring their experiments to life. The SpectraMax iD3s and iD5e readers are more than instruments—they’re extensions of your scientific imagination.
Whether you're designing a straightforward ELISA or a multi-layered live-cell assay masterpiece, the NEW SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers deliver the flexibility and performance today’s research demands. The iD3s reader offers a streamlined, affordable platform with a tunable monochromator and temperature control, while the iD5e reader adds enhanced detection modes including time-resolved fluorescence (TRF) and tunable fluorescence polarization (FP) and can be expanded to include TR-FRET, HTRF, BRET and western blot. Both readers support onboard fluidics with optional dual injectors.
For live-cell assays and microbial growth experiments, environmental for CO₂/O₂ regulation and advanced shaking are available to maintain ideal growth conditions. Data acquisition and analysis are driven by SoftMax® Pro Software—the most cited microplate reader software in the industry.
Crafted for the Canvas of Modern Science
Supports diverse assay needs
Perform ELISA, nucleic acid and protein quantitation, cell viability, microbial assays, western blot, and more.
Simplified setup & analysis
Get reproducible, publication-ready results using SoftMax® Pro Software with 200+ prewritten protocols and simultaneous data acquisition and analysis.
Fast, consistent mixing for high-performance flash assays
Dual injectors available for fast kinetic assays with SmartInject® Technology, ensuring complete reagent mixing in every well for high-precision with 10 µL dead volume.
Why choose the NEW SpectraMax iD series
SpectraMax® iD3s reader: Streamlined power for everyday assays
- 3 detection modes: absorbance, fluorescence, and luminescence
- CO₂/O₂ control and shaking options for live-cell support
- Dual injectors for fast, high-precision reagent mixing
- Tunable monochromator with 1-nm wavelength increments for full wavelength flexibility
SpectraMax iD5e reader: Advanced detection for complex assays
- 5 detection modes including TRF and fluorescence polarization
- Dual injectors for fast, high-precision reagent mixing*
- CO₂/O₂ control and advanced shaking options for live-cell support*
- TR-FRET, HTRF®, and bottom-read luminescence*
- Hybrid optics for monochromator flexibility + filter-based sensitivity
- Western blot detection with femtogram sensitivity*
* Optional features
Built for everyday and advanced assays
From ELISA and nucleic acid quantification to live-cell and microbial assays, the SpectraMax® iD Series adapts to your evolving research needs.
Simplified data acquisition and analysis
Over 200 preconfigured protocols are available for a wide variety of common assays, including protocols for our entire suite of reagents. SoftMaxPro Software integrates data acquisition and analysis into a single, user-friendly interface.
Continuous support & service
Remote and onsite PhD level support is offered fortechnical and application related topics.
Start Your Next Scientific Masterpiece
Talk to our experts to explore other microplate reader options.
SpectraMax® i3x Multi-Mode Microplate Reader
Modular detection, cell imaging, and a wide range of essential assays as well as advanced assays like HTRF, FP, and Western blot—expanding your capabilities on a single platform.
SpectraMax® M-Series Multi-Mode Microplate Readers
Trusted performance across absorbance, fluorescence, luminescence, TRF, FP, and HTRF assays. With built-in spectral scanning, cuvette support, and seamless integration with SoftMax® Pro Software.
SpectraMax® Mini Multi-Mode Microplate Reader
Fast and compact absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation without the use of filters
Customer Success Stories
National Horizons Centre uses the SpectraMax iD5 & SoftMax Pro Software to train the next generation of scientists
The NHC team chose a SpectraMax iD5 Multi-Mode Microplate Reader, which enables researchers to measure a variety of assays including absorbance, fluorescence and luminescence, as well as TRF and FP. SoftMax Pro 7 Software comes with a massive library of preconfigured protocols including plate layout and subsequent analyses that expedite the workflow and ensure reproducibility of results.
RiboPro use the SpectraMax iD3 to advance research into life-changing mRNA therapeutics
The team used the SpectraMax iD3 Multi-Mode Microplate Reader to perform luciferase assays to determine the performance of new LNP formulations. They also use it for cell viability assays and immunogenicity ELISAs to determine the (reduced) toxicity of both the mRNA and LNP formulations, as well as to measure the concentrations of RNA and DNA during their mRNA synthesis process. As such, it has become a crucial piece of equipment for the group. In the photo above, Marc Penders of RiboPro is using the SpectraMax iD3 for a lipid oxidation assay..
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