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Confidence Built In: For Every Lab, Every Assay

SpectraMax® i Series Multi-Mode Microplate Readers deliver trusted, decision-ready data with the flexibility, performance, and support your lab needs to grow

The SpectraMax® i Series of multi‑mode microplate readers - iD3s, iD5e, and i3x - are designed to generate high‑quality data for everyday assays such as DNA, RNA, and protein quantification, ELISAs, cell viability, and luciferase reporter assays, as well as complex, multi‑parameter workflows including multiplexed TR‑FRET and AlphaScreen® assays, live‑cell kinetic studies, and cytotoxicity assays. With over 85,000 scientific citations and four decades of trusted performance and partnership in research and regulated labs, the SpectraMax i-Series gives you the flexibility to adapt as your needs evolve. Whether you're accelerating discovery, ensuring QC consistency, or preparing for regulatory compliance, SpectraMax readers, paired with powerful, GxP-ready SoftMax® Pro Software, offer a future-ready platform that grows with your lab.

Why Scientists Around the World Choose SpectraMax Readers

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Most cited microplate reader platform 

With over 85,000 peer-reviewed publications citing SpectraMax® readers and SoftMax® Pro software, this is the most trusted microplate platform in life science research.
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Compliance-ready when needed

SoftMax® Pro Software offers flexible options for every lab, from streamlined data analysis to full 21 CFR Part 11 compliance with audit trails, e-signatures, and validation tools for regulated environments.
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Expert support and decades of partnership

Our scientists and support teams bring 40+ years of experience helping research and regulated labs succeed. From installation to optimisation, we’re here to guide your team every step of the way.

Ready to find the right fit for your lab?

With over  85,000 scientific citations, PhD level support, and  40+ years of experience, SpectraMax® readers are trusted by researchers worldwide for reliable, flexible performance.


Now through our limited-time offer, you can save up to 20% on SpectraMax® i Series Readers.

Get Your Personalised Quote & Product Info

One Platform - Endless Applications

From core absorbance, fluorescence, and luminescence assays to advanced techniques like TR‑FRET, AlphaScreen®, live‑cell kinetics, and imaging‑based workflows enabled with the optional SpectraMax® MiniMax™ 300 Imaging Cytometer, the SpectraMax® i Series brings a broad range of applications and read modes together on a single, scalable platform, so you can run today’s assays with confidence and expand your research without adding complexity or new instruments.

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SoftMax Pro Software: The Ultimate Microplate Data Acquisition and Analysis Tool

SoftMax® Pro Software is the most published microplate reader acquisition and analysis software. Compatible with Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data acquisition and analysis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Every step is optimised for data acquired from a Molecular Devices microplate reader or data imported from another source to simplify analysis and reporting. Compliance tools are available for regulated laboratories providing end-to-end chain of custody.

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Preconfigured and Customisable Protocols

SoftMax Pro Software offers a blend of prewritten protocols with included acquisition settings and the flexibility to set custom experiment parameters, ensuring streamlined data measurement and analysis.

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Versatile Data Import and Analysis

The software allows the import of raw data in microplate format from any scientific instrument. It provides consistent methods for data evaluation, reducing errors and simplifying the analysis of complex data.

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Professional Reporting and Publishing Tools

Equipped with features for creating flexible data output formats, the software enables users to publish reports efficiently. It supports various formats like lab notebook layouts, PDFs, Excel files, and XML for LIMS integration, enhancing the ease of documentation and publication

Real Labs. Real Results.

Poly-Dtech team at facility in Strasbourg

Joan Goetz, PhD |
Poly-Dtech

Enabling Scalable Immunoassays

 

Poly-Dtech use the SpectraMax iD5 to develop high‑sensitivity TR-FRET and multiplex immunoassays under customer‑identical conditions, favouring the reader for its reproducibility and flexibility.

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Luis Gimenez-Lirola, PhD |
Iowa State University

Advancing Vaccine Research

Dr. Luis Gimenez-Lirola’s Lab, at Iowa State University in the College of Veterinary Medicine is using the SpectraMax i3x to developed assays for automated screening, and analysis of neutralizing antibody assays.

 

Real Scientists. Real Impact.

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Application Highlights

Reliable ELISA and Protein Quant Spectramax i3x

Reliable ELISA & Protein Quantitation

Confident results for everyday and regulated workflows
 
  • Perform sandwich and competitive ELISAs with high sensitivity
  • Tunable monochromators and hybrid optics for assay flexibility
  • Ideal for cytokine profiling, biomarker validation, and QC labs
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Live‑Cell & Cell Viability Assays

Measure real‑time cellular responses with confidence.

 

  • Support fluorescence and luminescence‑based viability assays
  • Optional CO₂/O₂ control and advanced shaking for live‑cell workflows
  • Suitable for cytotoxicity, proliferation, and pathway analysis
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Multiplexed & Advanced Detection Assays

One platform for evolving assay complexity

  • Perform TRF, fluorescence polarization, and multiplexed assays (iD5e/i3x)
  • Expand into TR‑FRET, HTRF®, and western blot detection as needs grow
  • Reduce instrument sprawl while future‑proofing your lab

Ready to find the right fit for your lab?

With over  85,000 scientific citations, PhD level support, and  40+ years of experience, SpectraMax® readers are trusted by researchers worldwide for reliable, flexible performance.


Now through our limited-time offer, you can save up to 20% on SpectraMax® i Series Readers.

Get Your Personalised Quote & Product Info

Molecular Devices: A life science company

270,000+

CITATIONS ACROSS OUR
PORTFOLIO

160,000+

SOLUTIONS IN LABS
WORLDWIDE

40

HARDWARE & SOFTWARE
TECHNOLOGY PLATFORM

A trusted life science partner to researchers worldwide

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*Terms and Conditions Appl
Terms and conditions:

Purchase order must reference promotion code 2026MDBIOPRO. The offer is to purchase the SpectraMax iD3s, iD5e, or i3x Multi-Mode Microplate Reader for up to 20% off list price. Valid August 01, 2025 through May 29, 2026. The offer does not include shipping and handling, installation or training. The offer does not include any other options or accessories. This promotion cannot be combined with any other promotions or discounts. No substitutions or swap outs. The discount is not convertible to cash. Promotion is not valid for standing orders. All prices exclude delivery charges and local taxes, which are the responsibility of the customer. Promotion is available in the United States and Canada, to new customers only, when buying directly from Molecular Devices. All products are for research use only. Void where prohibited. The purchase is subject to Molecular Devices standard terms and conditions of sale. https://www.moleculardevices.com/terms-and-conditions.​

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